AGO1 antibody, rabbit polyclonal

Background

Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that

AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in

post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its

action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin

and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing

suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.

Other names: AGO1, ARGONAUTE 1, ATAGO1, ICU9

Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein

containing the C-terminal region of Arabidopsis thaliana AGO1 (AT1G48410). Antibodies were purified by

affinity purification using immunogen.

Concentration: 1.0mg/ml

Tested applications: Western blot (WB), IF

Expected/apparent MW: 116/130 kDa

Species specificity: Arabidopsis thaliana

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.