PIN1 antibody, rabbit polyclonal

Background

Encodes an auxin efflux carrier involved in shoot and root development. It is involved in the maintenance of

embryonic auxin gradients. Loss of function severely affects organ initiation, pin1 mutants are characterised by an

inflorescence meristem that does not initiate any flowers, resulting in the formation of a naked inflorescence stem.

PIN1 is involved in the determination of leaf shape by actively promoting development of leaf margin serrations.

In roots, the protein mainly resides at the basal end of the vascular cells, but weak signals can be detected in the

epidermis and the cortex. Expression levels and polarity of this auxin efflux carrier change during primordium

development suggesting that cycles of auxin build-up and depletion accompany, and may direct, different stages of

primordium development. PIN1 action on plant development does not strictly require function of PGP1 and

PGP19 proteins.

Other names: ARABIDOPSIS THALIANA PIN-FORMED 1, ATPIN1, PIN-FORMED 1, PIN1 AT1G73590

Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein

containing the C-terminal region of arabidopsis thaliana PIN1 (AT1G73590). Antibodies were purified by affinity

purification using immunogen.

Concentration: 1.0mg/ml

Expected/apparent MW: 67 kDa

Species specificity: Arabidopsis thaliana

Tested applications: Western blot (WB), IF

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY